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KMID : 0371619990150020147
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Journal of Wonkwang Medical Science
1999 Volume.15 No. 2 p.147 ~ p.154
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Effect of Nerve Growth Factor on Cultured Rat Schwann Cells in Hyperglycemic Condition
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Kim Kyoung-Hee
Jang Geun-Young
Baek Seung-Hoon
Hyoung Keon-Young
Cho Chung-Gu
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Abstract
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Background: Nerve growth factor(NGF) is produced in tissues innervated by its responsive neurons. In the peripheral nervous system, NGF messenger RNA(mRNA) is produced in target fields of small pain and temperature- mediating dorsal root ganglia(DRG) sensory neurons and sympathetic neurons. NGF receptors are expressed in these neurons. NGF has been shown to promote their survival differentiation, and maitenance. But the mechanism of neuronal damage in diabetes and the effect of NGF on diabetic neuropathy are not clear.
Methods: In order to clarify the neurotoxic effect of hypergiycemia, the hyperglycemia-induced cytotoxic effects were evaluated by MTT assay on cultured rat Schwann cells which were grown with media containing concentrations of high glucose for inducing hyperglycemic condition. And also the neuroprotective effect of nerve growth factor(NGF) against hyperglycemia- induced neurotoxicity were also examined.
Results: 1. MTT50 value was at concentration of 25mM glucose after Schwann cells were exposed to various concentrations of glucose for 72 hours. 2. Cell viability of cultured rat Schwann cells treated with hyperglycemic media made with 25-35mM glucose was markedly decreased in a dose-dependent manner compared with control medium(normoglycemic medium) containing concentration of 5.5mM glucose, While cell number did not show a dosedependent decrease. 3. Cultured Schwann cells exposed to hyperglycemic medium made with 25mM glucose for 72 hours did not show any morphological change as well as decrease of cell number. 4. Pretreatment of 10ng/㎖ NGF for 2 hours increased remarkably the cell viability of cultured Schwann cells exposed to hyperglycemic medium made with 25mM glucose for 72 hours.
Conclusions: From the above results, it is suggested that hyperglycemic condition induces the decrease of cell viability on cultured Schwann cells of rat. But it did not show the decrease of cell number and morphological change. And also selective neurotrophic factors such as NGF are very effective in preventing dysfunction of cells induced by glucose toxicity in hyperglycemic condition.
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KEYWORD
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Nerve growth factor, Diabertic neuropathy, MTT assay, Schwann cell
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